Antibody Kinetic Assays

What is Antibody Kinetic Assay?

It is well known that antibody kinetic assays have gained widespread recognition in the realm of biotechnology, primarily because of their effectiveness in measuring the binding affinity and specificity of antibodies to their target antigens. These assays are capable of determining the rate at which antibodies bind to their respective antigens as well as the rate at which they dissociate from them. By generating pertinent data about the duration and intensity of interactions between the antibody and the antigen, the assay has become an indispensable tool for antibody development, as it can identify antibodies that exhibit superior potency and specificity, making them ideal for therapeutic purposes.

Notably, the antibody kinetic assay is not limited to its traditional role but can be utilized in various applications, including epitope mapping, antibody screening, and optimization of antibody conjugation. It's versatile nature is a testament to its significance in the field of biotechnology.

Surface plasmon resonance (SPR) sensorgramsSurface plasmon resonance (SPR) sensorgrams (Gjetting et al., 2019)

Antibody Kinetic Assay in Creative Proteomics

Creative Proteomics is proud to offer a diverse array of advanced technology platforms to support your antibody kinetic analysis needs. Our cutting-edge platforms include the highly acclaimed surface plasmon resonance (SPR), bio-layer interferometry (BLI), and micro-scale thermophoresis (MST) techniques.

Technique Surface Plasmon Resonance (SPR) Biolayer Interferometry (BLI) Microscale Thermophoresis (MST)
Labeling Label-free Label-free Label-free
Principle Changes in refractive index Changes in interference pattern Changes in movement due to temperature
Kinetic Parameters Measured ka, kd, KD ka, kd, KD ka, kd, KD
Advantages High sensitivity, real-time measurement capabilities, ability to detect small molecule interactions Low sample consumption, high sensitivity, ability to measure a wide range of biomolecular interactions Ability to measure weak interactions, compatible with a wide range of biomolecules

Applications of Antibody Kinetic Assay

Antibody Discovery and Development: Evaluate the binding affinity and kinetics of potential therapeutic antibodies and identify lead candidates for further development. By measuring kinetic parameters such as binding rate constant (ka), dissociation rate constant (kd) and equilibrium dissociation constant (KD), candidate antibodies can be evaluated for their potential and optimized for clinical use.

Quality Control: Assessing the purity and quality of antibody formulations provides data on binding kinetics and helps ensure the consistency and quality of antibody batches.

Biosimilar Development: Antibody kinetic assays are used to compare biosimilars to their reference products to ensure they have the same clinical efficacy and safety as their reference products.

Immunoassay development: Optimizing immunoassays by measuring the binding kinetics of antibodies to their target antigens helps develop more sensitive and specific immunoassays that can detect low levels of target analytes with high accuracy.


  1. Gjetting, Torben, et al. "Sym021, a promising anti-PD1 clinical candidate antibody derived from a new chicken antibody discovery platform." MAbs. Vol. 11. No. 4. Taylor & Francis, 2019.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.