Surface Plasmon Resonance (SPR)

Surface Plasmon Resonance (SPR)

Introduction

Surface plasmon resonance (SPR) has been widely used in many fields because of its unique specificity, sensitivity, quantification, and no need to label the target molecule (label-free). In this technology, the ligand is immobilized on the surface of the metal film. When the analyte flows in the microfluidic channel and interacts with these ligands, SPR can monitor the reflected light intensity to determine the amount of the analyte or track the resonance angle shifts. So far, due to the accuracy and convenience of SPR, it has not only been used to study the interaction of proteins, peptides, small molecules, bacteria, etc. but also various types of SPR-based biosensors have been developed for clinically relevant biomarker development, food safety (pathogens and toxins), environmental monitoring (pollutants), etc.

Pictorial representation of the SPR principle - Creative ProteomicsFigure 1. Pictorial representation of the SPR principle - Creative Proteomics

Services

Creative Proteomics recruited a large number of technicians and experts with rich experience in SPR, and introduced the world-class Biacore™ SPR instruments to establish this technology platform with high-throughput compound screening capabilities and extraordinary detection sensitivity.

  • We use advanced Biacore™ T200 and Biacore™ 8+ equipment for data collection. The platform has a high throughput, and Biacore™ 8K+ can screen more than 2000 small molecule fragments per day.
  • On this platform, customers can accurately and efficiently identify and analyze the interactions between various molecules, as well as high-throughput fragment screening, binding specificity analysis, kinetics and affinity determination, and antibody characterization, etc. The flexibility and high throughput of the platform can also support customers in drug discovery for complex targets.

Schematic of a SPR sensorgramFigure 2. Schematic of a SPR sensorgram (Thoren, K. 2019)

Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Highlights

  • High-information, can provide kinetic information as well as affinity and potency data
  • Able to test the influence of various co-factors on the analyte-target interaction
  • Capable of detecting weak affinity interactions of low molecular mass fragment compounds
  • Ability to select the best drug candidates by kinetic selective screening of drug analogs
  • Sensitive and high throughput

Creative Proteomics is an international biotechnology company dedicated to the study of molecular interactions and other related fields. We have introduced advanced Biacore™ SPR instruments to establish a multi-functional and highly sensitive surface plasmon resonance technology platform, which can help customers to more efficiently study the interaction between proteins, peptides, small molecules, etc. Our one-stop service is designed to save customers time and money.

Reference

  1. Thoren, K. The role of surface plasmon resonance in clinical laboratories. AACC. 2019.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.

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