RAP-MS Service

RNA-protein interactions play a key role in many cellular processes, including gene regulation, protein synthesis and cellular signalling pathways. RNA antisense purification coupled with mass spectrometry (RAP-MS) is a cutting-edge technology used for the study of RNA-protein interactions in cells. The main idea behind RAP-MS is to capture RNA molecules and their interacting partners (such as RNA-binding proteins), and then identify them using mass spectrometry.

What RAP-MS can offer?

• Study of gene regulation: Study the interactions between RNA-binding proteins and messenger RNA (mRNA) molecules, providing insight into the mechanisms of gene regulation.
• Identification of non-coding RNA-protein interactions: Study the interactions between non-coding RNA molecules, such as microRNAs, and their target proteins, providing insight into the role of non-coding RNA in cellular processes.
• Study of RNA metabolism: Study the interactions between RNA-binding proteins and RNA molecules involved in RNA metabolism, such as spliceosomal RNAs and ribosomal RNAs, providing insight into the molecular mechanisms of RNA metabolism.
• Study of RNA-protein interactions in disease: RAP-MS can be used to study the interactions between RNA-binding proteins and RNA molecules in disease states, such as cancer, providing insight into the molecular mechanisms of disease and potential targets for therapeutic intervention.
• Study of the role of RNA-protein interactions in development: Study the interactions between RNA-binding proteins and RNA molecules during development, providing insight into the molecular mechanisms of development and the role of RNA-protein interactions in the process.

Creative Proteomics offers an RNA-Protein interaction analysis service based on RAP-MS technology.

Principle of RAP-MS

The principle of RAP-MS involves the use of antisense RNA oligonucleotides to selectively capture target RNA molecules in cells. Antisense RNA oligonucleotides are short, single-stranded RNA sequences that are complementary to the target RNA sequence. When these antisense oligonucleotides are added to cells, they bind to the target RNA molecules, forming RNA-antisense complexes. These complexes can then be isolated and purified using various techniques, such as immunoprecipitation, and then analyzed by mass spectrometry to identify the proteins associated with the target RNA.

Routine Analytical Procedures for RAP-MS

Advantages of RAP-MS

High specificity: RAP-MS uses antisense RNA oligonucleotides to specifically capture target RNA molecules, providing high specificity for the identification of RNA-protein interactions.

High sensitivity: Mass spectrometry allows for the identification and quantification of even low-abundance components of RNA-protein interactions, providing high sensitivity for the detection of rare or transient interactions.

Multiplexing capability: RAP-MS allows for the simultaneous analysis of multiple RNA-protein interactions, providing the ability to study complex RNA-protein networks in a single experiment.

High-throughput capability: RAP-MS can be automated and scaled up to allow for high-throughput analysis of RNA-protein interactions, providing the ability to study large numbers of interactions in a relatively short amount of time.

Integrative approach: RAP-MS provides both the identification and quantification of the components of RNA-protein interactions, providing an integrative approach for the study of these interactions.

Direct measurement of interactions: RAP-MS provides direct measurement of RNA-protein interactions, avoiding the need for indirect methods that may introduce biases or inaccuracies.

Large dynamic range: Mass spectrometry has a large dynamic range, allowing for the measurement of both low- and high-abundance components of RNA-protein interactions in the same experiment.

Creative Proteomics has accumulated a wealth of experience in RNA-protein interaction research. Our team of technical professionals also offers other techniques for RNA-protein interaction analysis, including ChIRP, CHART, RAP-MS, RIP and RNA Pull Down, etc. Contact us to learn more.