Ex Situ Binding Assay of Virus-Host Interactions

Ex Situ Binding Assay of Virus-Host Interactions

Background

The interactions between virus and host play an important role in every stage of the virus life cycle, including infection of host cells, replication of the viral genome, and assembly of virus particles. The analysis of virus-host interaction is an important field of biology, which can clarify key pathways such as immunity, cell signal transduction, replication, and division. Besides, the identification of potential virus-host interactions is very useful for controlling diseases caused by a variety of viruses and is the key to helping scientists develop new drugs to treat viral infections. For example, the drug maraviroc inhibits the entry of HIV by binding to the cellular receptor (CCR5) and preventing its binding to the viral glycoprotein (gp120).

Currently, many methods can be used for virus-host interaction analysis to help us explore the interaction network, but what we need to know is that no matter which method, there are limitations in a specific environment. Therefore, it is necessary to combine multiple methods for the study of these interactions. Before designing an experiment, we should fully understand the nature of the experiment and conduct a comprehensive analysis.

For HIV-1, gp120/CCR5 binding complexes mediate the formation of the contact zoneFig 1. For HIV-1, gp120/CCR5 binding complexes mediate the formation of the contact zone (Sun, S.X.; Wirtz, D. 2006)

Services

Ex situ binding experiments of virus-host interaction can be used to analyze interactions that do not occur in the host cell, but non-host cell or solution. Creative Proteomics provides yeast two-hybrid, GST pull-down and other ex situ experimental methods to help customers explore these interactions, and after the experiment, we will add in situ confirmation steps or designs other ex situ experiments used for testing to verify the results.

Customers can choose a certain technology according to the needs of the project, or contact us directly for consultation, and our expert team will provide you with a customized experimental program.

Applications

  • Virus-host interaction research
  • Pathogenic mechanism research
  • Virus life cycle research
  • Antiviral drug development
  • Develop new avenues of patient care

Highlights

  • Fast and highly accurate
  • Able to screen large numbers of bait and prey combinations simultaneously
  • Experimental guidance provided by experienced experts
  • Tailored experimental program  

Creative Proteomics has been focusing on virus-related research for more than ten years and has recruited a large number of professional technicians and analysts in this field. The virus-host interaction ex situ analysis service we provide can achieve high accuracy, high sensitivity, cost-effective, and provide customers with strong technical support. We are honored to be your competent research assistant.

References

  1. Sun, S.X.; Wirtz, D. Mechanics of enveloped virus entry into host cells. Biophysical Journal. 2006, 90(1): L10-L12.
  2. Gillen, J.; Nita-Lazar, A. Experimental analysis of viral-host interactions. Frontiers in Physiology. 2019, 10.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.