Reverse Mammalian Protein-Protein Interaction Trap (Reverse MAPPIT)

Reverse Mammalian Protein-Protein Interaction Trap (Reverse MAPPIT)

Introduction

Proteins are the executors of most life activities, and the interaction between proteins is the core of cell mechanisms. Therefore, changes in the interaction caused by abnormal protein expression patterns or mutations will trigger cell dysfunction and ultimately lead to diseases. In addition, many viral and bacterial pathogens rely on protein-protein interactions to exert their destructive effects.

The Reverse Mammalian Protein-Protein Interaction Trap (Reverse MAPPIT) is a mammalian reverse two-hybrid technology. This method is a two-hybrid complementary system modified by the forward MAPPIT technology, in which a positive readout is generated when the protein-protein interaction is interrupted. The interaction between bait and inhibitory prey (i-prey) blocks signaling and reporter induction. Only through competing proteins or small organic molecules disrupting their interactions can the signaling be restored, leading to a positive readout. With the help of an effective screening platform, this method can be applied to screen for interferences of specified protein-protein interactions in intact mammalian cells. In human cells, it can analyze the destruction, toxicity, and permeability of the test compounds, and is suitable for screening protein-protein interactions of interest in targeted therapy or mapping interaction interface between proteins.

Schematic view of the forward MAPPIT and reverse  MAPPIT principlesFigure 1. Schematic view of the forward MAPPIT and reverse MAPPIT principles (Eyckerman, S.; et al. 2005)

Services

Creative Proteomics has more than ten years of successful experience in MAPPIT and reverse MAPPIT applications. Our powerful technical platform and professional technical personnel are the guarantees of our high-quality services. The reverse MAPPIT experiment we provide includes but is not limited to the steps of bait and prey constructs generation, cell culture, reporter assays, western blot analysis, and co-immunoprecipitation. Our experts can also customize the experimental program according to the different needs of customers.

Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Highlights

  • Topological flexibility
  • High throughput
  • Omit internal control of cell toxicity
  • Reliable, efficient, and cost-effective
  • Limit false positive signals
  • No require production and purification of the interacting protein partners

Creative Proteomics is an international biotechnology company dedicated to research in molecular interactions and other related fields. The reverse mammalian protein-protein interaction trap platform we constructed has the characteristics of high quality and efficiency, and the data obtained can be directly used for paper publication. Our one-stop service aims to save customers time and money.

References

  1. Eyckerman, S.; et al. Reverse MAPPIT: screening for protein-protein interaction modifiers in mammalian cells. Nature Methods. 2005.
  2. Lemmens, I.; et al. Reverse MAPPIT detects disruptors of protein-protein interactions in human cells. Nature Protocols. 2006.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.

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