Crosslinking Protein Interaction Analysis

Crosslinking Protein Interaction Analysis

Introduction

Crosslinking Protein Interaction Analysis Platform

Protein-protein interaction is an important form of its function. However, since most protein-protein interactions are transient, it becomes more difficult to study these PPIs. The crosslinking method provides great help for today's interaction research and proteomics research, that is, capturing or freezing these transient contacts to study which proteins are involved and how they interact. In this method, crosslinking reagents are used to covalently bond the proteins together as they interact, and then the protein-protein complex is captured and studied. Common functional groups on crosslinkers can freeze transient interactions in place, or fix weakly interacting molecules in a sufficiently stable complex for better separation and characterization.

Crosslinking can be carried out in vivo and in vitro. In vivo crosslinking can capture the interactions between proteins in their natural environment, thereby reducing false positive interactions and avoiding the loss of interactions during cell lysis. In vitro crosslinking is more practical for researchers who want to modify proteins, and In vitro crosslinking can better target specific crosslinking events, because many reaction conditions such as the concentration of reactants, the purity of the target protein, ion strength, temperature and pH can be controlled during in vitro research.

Services

At present, Creative Proteomics' crosslinking analysis platform has been established for more than 20 years, and the platform is jointly managed by our experienced biologists and chemists. We provide a variety of different types of crosslinking reagents to meet customers' research needs, including different functional groups, short and long spacer arm length, cleavable and non-cleavable, hydrophilic and hydrophobic, homobifunctional or heterobifunctional. In addition to chemical crosslinking, our photoreactive crosslinking is also available for customers to choose from.

Our service is one-stop, providing all the steps involved in the project, including:

  • Experimental design
  • Crosslinking reagent selection
  • Sample preparation
  • Parameter optimization
  • Cell lysis
  • Analysis experiments (electrophoresis, WB, Co-IP, XL-MS)
  • Report delivery

Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Highlights

  • Capturing transient interactions
  • Stabilize weak interactions
  • Proteins can be modified artificially
  • Can target specific crosslinking events
  • High accuracy
  • Cost-effective

Creative Proteomics is an international biotechnology company dedicated to research in molecular interactions and other related fields. We have rich experience in crosslinked protein interaction analysis and can use crosslinking methods to help customers better study the interactions between molecules, especially the transient interactions. Our one-stop service aims to save customers time and money.

Reference

  1. Yakovlev, A.A. Crosslinkers and their utilization for studies of intermolecular interactions. Neurochemical Journal.2009, 3(2): 139-144.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.

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