Immunoprecipitation

Immunoprecipitation

Introduction

Immunoprecipitation (IP) is a classic method for studying the interaction between protein and protein, protein and nucleic acid based on the specific interaction between antigen and antibody. When the cell is lysed under non-denaturing conditions, many protein-protein and protein-nucleic acid interactions in the intact cell are retained. If there is an XY complex in the cell, immunoprecipitate X with antibodies against X protein (also called bait protein), then protein Y or nucleic acid Y bound to X in the cell is also precipitated. Therefore, the antibody of X is added to the cell lysate to precipitate protein X, and then analysis whether Y is also present in the precipitate. The advantage of this method is that this interaction is completed in the cell, which can best reflect the interaction in the natural state, so the result is more reliable.

Services

Creative Proteomics is a biotechnology company that focuses on the study of molecular interactions. We have organized numerous biochemical researchers and established a mature and advanced immunoprecipitation platform. In our platform, customers can study protein-protein and protein-nucleic acid interactions in depth. We provide several technologies including but not limited to the following.

We provide highly sensitive and specific immunoprecipitation related reagents and services to study protein-protein interactions. In follow-up experiments, many methods such as west blot and mass spectrometry can be used to help customers analyze known and unknown interactors.

Diagrammatic representation of the co-immunoprecipitation (co-IP) procedureFigure 1. Diagrammatic representation of the co-immunoprecipitation (co-IP) procedure (Lin, J.S.; Lai, E.M. 2017)

The technology to study the binding of RNA and protein in cells is a powerful tool for understanding the dynamic process of post-transcriptional regulatory networks. RIP technology uses antibodies against the target protein to precipitate the corresponding RNA-protein complexes and analyze them by qPCR, microarray, or sequencing.

Diagrammatic representation of the RNA binding protein immunoprecipitation (RIP) procedureFigure 2. Diagrammatic representation of the RNA binding protein immunoprecipitation (RIP) procedure (Head, S.R.; et al. 2014)

  • Crosslinking Immunoprecipitation (CLIP)

This is a molecular biology method that uses UV cross-linking combined with co-immunoprecipitation to analyze protein-RNA interactions or precisely locate RNA modifications. We also provide a variety of advanced derivative technologies such as CLIP-SEQ, PAR-CLIP, iCLIP and eCLIP.

Schematic representation of the iCLIP protocol Figure 3. Schematic representation of the iCLIP protocol (Konig, J.; et al. 2011)

  • Chromatin Immunoprecipitation (ChIP)

ChIP is to randomly shear DNA along with bound proteins into small fragments, and then precipitate the complex by immunological methods to specifically enrich DNA fragments bound by the target protein. Reverse the cross-linking to release the DNA and digest the proteins, information about the interaction between protein and DNA can be obtained. We also provide a variety of analysis methods including qPCR, ChIP-seq, microarray or NGS, PCR and ChIP-chip are also options for downstream analysis.

Diagrammatic representation of the chromatin immunoprecipitation (ChIP) procedureFigure 4. Diagrammatic representation of the chromatin immunoprecipitation (ChIP) procedure (Mukhopadhyay, A.; et al. 2008)

Customers can choose different technologies and equipment according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Highlights

  • High precision
  • Professional technicians, advanced platform
  • Short experiment period
  • Data can be directly used for article publication
  • Cost-effective

Creative Proteomics is an international biotechnology company dedicated to the study of intermolecular interactions and other related fields. In order to help customers study protein and nucleic acid related interactions, we have established a powerful immunoprecipitation platform and added a variety of classic and emerging technologies. Customers can complete a large number of advanced experiments and projects on our platform.  

References

  1. Lin, J.S.; Lai, E.M. Protein-Protein Interactions: Co-Immunoprecipitation. Bacterial Protein Secretion Systems. 2017.
  2. Konig, J.; et al. iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution. Journal of Visualized Experiments. 2011.
  3. Head, S.R.; et al. Library construction for next-generation sequencing: overviews and challenges. Biotechniques. 2014, 562(2).
  4. Mukhopadhyay, A.; et al. Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans. Nat Protoc. 2008, 3(4): 698-709.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.

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