Nucleic Acid-Related Interaction Analysis

What are Nucleic Acid Interactions?

Nucleic acid (DNA/RNA) is an indispensable component of all known life forms. It is widely found in all plants, animals and microbial cells. It is responsible for the storage and replication of genetic material, as well as protein synthesis. Nucleic acids can also interact with many molecules to form the basis of life activities such as growth, reproduction, heredity and metabolism. Nucleic Acid Interactions refer to the binding and interactions between nucleic acid molecules (DNA and RNA) and other molecules (such as proteins, small compounds, etc.). These interactions play a critical role within living organisms, influencing cellular survival, development, reproduction, metabolism, and more. Nucleic acids regulate gene expression, RNA processing, protein synthesis, cellular signaling, and numerous other essential processes through their interactions with various molecules.

Why Analyze Nucleic Acid Interactions?

Deciphering Biological Function: Interactions between nucleic acids, proteins, and small molecules directly influence the biological functions of these molecules. Analyzing these interactions provides insights into gene regulation, protein synthesis, RNA processing, and cellular signaling, among other key biological processes.

Unveiling Disease Mechanisms: Aberrant nucleic acid interactions are closely linked to the development of various diseases. Analyzing these interactions helps uncover molecular mechanisms underlying diseases, offering potential new targets and strategies for diagnosis and treatment.

Drug Development: Nucleic acid interactions are pivotal in drug development. Many drugs interfere with nucleic acid interactions to treat diseases. A deeper understanding of these interactions guides drug design and development.

Evolution and Adaptation: Nucleic acid interactions shed light on the evolutionary and adaptive processes of organisms. Analyzing these interactions reveals how different organisms adapt and evolve in various environments.

Fundamental Research: Nucleic acid interactions are foundational to life science research. Analyzing these interactions provides insights into cellular mechanisms, signaling networks, and the transmission and regulation of genetic information.

Fig 1. DNA structure (Minchin, S.; Lodge, J. 2019)

Nucleic Acid-Related Interaction Analysis Platform at Creative Proteomics

DNA-RNA Interaction Analysis:

This project focuses on deciphering interactions between DNA and RNA molecules. By utilizing advanced techniques, it provides insights into gene expression regulation, RNA processing, and potential non-coding RNA functions.

• Chromatin Immunoprecipitation Sequencing (ChIP-seq)
• RNA Immunoprecipitation Sequencing (RIP-seq)
• UV Cross-Linking and Immunoprecipitation (CLIP-seq)
• Electrophoretic Mobility Shift Assay (EMSA)

RNA-RNA Interaction Analysis:

Explore intricate interactions between diverse RNA molecules. This project helps uncover the roles of RNA in post-transcriptional regulation, RNA-protein complexes, and the potential impact of RNA-RNA interactions on cellular processes.

• Cross-Linking and Immunoprecipitation Sequencing (CLIP-seq)
• RNA-Binding Protein Sequencing (RBP-seq)
• PAR-CLIP
• RNA Co-Immunoprecipitation (RNA Co-IP)

RNA-DNA Interaction Analysis:

Investigate the dynamic interactions between RNA and DNA. By analyzing RNA-DNA associations, this project sheds light on chromatin-associated RNA function, epigenetic regulation, and potential regulatory elements.

• DNA-RNA Immunoprecipitation (DRIP-seq)
• RNA-DNA Annealing and RNA Immunoprecipitation Sequencing (RADAR-seq)
• MeRIP-seq
• Chromatin Isolation by RNA Purification (ChIRP)

Protein-DNA/RNA Interaction Analysis:

Study the intricate interplay between proteins and DNA/RNA molecules. This project provides valuable insights into transcriptional and post-transcriptional regulation, protein-DNA binding sites, and RNA-protein interactions.

• Chromatin Immunoprecipitation Sequencing (ChIP-seq)
• RNA-Binding Protein Sequencing (RBP-seq)
• MeDIP-seq
• DNA Affinity Chromatography

Non-coding RNA Functional Studies:

Uncover the roles of non-coding RNAs in gene regulation and disease. This project explores various non-coding RNA species, shedding light on their functions, interactions, and potential as diagnostic or therapeutic targets.

• Long Non-Coding RNA Sequencing (lncRNA-seq)
• Small RNA Sequencing (sRNA-seq)
• Small Nucleolar RNA Sequencing (snoRNA-seq)
• Circular RNA Sequencing (circRNA-seq)

DNA/RNA-Graphene Interactions:

Investigate interactions between DNA/RNA and graphene, a promising nanomaterial. This project explores potential applications in biosensing, drug delivery, and nanotechnology, leveraging cutting-edge techniques to characterize molecular interactions.

• Surface Plasmon Resonance (SPR)
• Atomic Force Microscopy (AFM)
• Quartz Crystal Microbalance (QCM)
• Graphene Oxide Pull-Down Assay

DNA/RNA-Drug Interactions:

Study how DNA/RNA molecules interact with drugs and therapeutic compounds. This project aids in drug discovery by providing insights into binding affinity, mode of interaction, and potential mechanisms of action.

• Drug Affinity Responsive Target Stability (DARTS)
• Microscale Thermophoresis (MST)
• Isothermal Titration Calorimetry (ITC)
• Ligand Affinity Chromatograph

DNA/RNA-Metal Ion Interactions:

Explore interactions between DNA/RNA and metal ions, uncovering the effects of metal coordination on nucleic acid structure and stability. This project contributes to our understanding of metal-mediated biological processes and potential applications in materials science.

• Metal-Induced Cleavage
• Spectroscopic Techniques (UV-Vis, Fluorescence, Circular Dichroism)
• EMSA with Metal Ions

Application of Nucleic Acid-Related Interaction Assay

Gene Regulation and Expression Insights: Understand the regulatory landscape by deciphering DNA-RNA interactions, revealing enhancer-promoter dynamics, and identifying key transcription factor binding sites.

RNA Processing and Splicing Mechanisms: Gain insights into RNA maturation and alternative splicing events through analysis of RNA-RNA and RNA-DNA interactions.

Epigenetic Landscape Decoding: Explore the interplay between DNA/RNA and epigenetic marks, unraveling the influence of non-coding RNAs on the epigenome.

Functions of Non-Coding RNAs: Uncover the roles of lncRNAs, sRNAs, and circRNAs in post-transcriptional regulation and disease processes.

RNA-Protein Interaction Networks: Dissect RNA-protein interactions to elucidate RNA metabolism, translation, and the assembly of RNA-protein complexes.

3D Genome Architecture Revealed: Investigate chromosomal interactions and higher-order genome structures for insights into spatial organization and gene regulation.

Precision Drug Discovery: Identify drug targets and mechanisms of action, guiding the development of therapeutics that target nucleic acid-protein interactions.

Disease Mechanism Understanding: Unearth aberrant nucleic acid interactions linked to diseases, paving the way for biomarker discovery and novel therapeutic strategies.

Cutting-Edge Nanotechnology: Explore DNA/RNA interactions with nanomaterials, such as graphene, for innovative biosensing and diagnostic applications.

Advancements in Biotechnology: Study DNA/RNA interactions with metal ions, contributing to the development of DNA-based materials and sensors.

Highlights

• Systematic technology platforms and powerful data analysis tools
• High sensitivity and high-throughput
• Ability to perform interaction analysis between multiple types of molecules
• Well-trained technicians and experienced experts
• Cost-effective

Creative Proteomics is a biotechnology company specializing in the study of molecular interactions. Our superior technology platforms and analysis tools can provide customers with powerful technical support, saving customers time and money while obtaining accurate data. We are honored to be your competent research assistant.