Protein-DNA interaction is the basic component of all biological systems and is the necessary basis for almost all biological processes, such as DNA replication, repair, transcription, regulation of gene expression, and packaging of chromosomal DNA. Proteins mainly interact with DNA through electrostatic interactions (salt bridges), dipole interactions (hydrogen bonds), and entropy effects (hydrophobic interactions). According to the different forces, they can be divided into two types: 1) sequence-specific binding and 2) sequence non-specific binding.
Understanding the mechanism of protein-DNA interaction in detail, determining which proteins are present in the protein-DNA complex, and identifying the nucleic acid sequence required for the process is essential for understanding the role of protein-DNA interactions in regulating cellular processes. Besides, any errors in the process of protein-DNA interactions may lead to serious consequences, and artificially interfering with these interactions with chemicals can control the expression of certain genes and provide new ideas for the treatment of many diseases. But so far, DNA-protein interactions are far from fully understood.
Fig 1. DNA-binding by IHF and T7 endonuclease I (Harteis, S.; Schneider, S. 2014)
Creative Proteomics integrates a large number of advanced technologies to form a systematic and mature protein-DNA interaction research platform, which can perform detailed detection and characterization of interactions in vivo and in vitro, each method has its own advantages and disadvantages under certain conditions.
The technologies we provide usually include but are not limited to DNase I footprinting, electrophoretic mobility shift assays (EMSA), bacterial one/two-hybrid systems (B1H/B2H). We also have developed many efficient and accurate chromatin immunoprecipitation (ChIP)-based technologies, such as chromatin immunoprecipitation with microarray (ChIP-chip), chromatin immunoprecipitation sequencing (ChIP-Seq), and chromatin immunoprecipitation-paired-end tags (ChIP-PET), etc. In addition, we have professional bioinformatics engineers who can provide customers with protein-DNA interaction predictions and molecular docking experiments.
Customers can choose a certain technology according to the needs of the project, or contact us directly for consultation, and our expert team will provide you with a customized experimental program.
Creative Proteomics has a lot of experience in protein-DNA interaction analysis. Our excellent technology platform has been continuously optimized and updated to ensure that we can provide customers with high-precision, high-resolution and time-saving services. We are honored to be your competent research assistant.