Protein-Carbohydrate Interactions

Protein-Carbohydrate Interactions

Background

Carbohydrates are everywhere, in addition to being part of the glycan structure of glycoproteins and glycolipids, carbohydrates also exist in the form of monosaccharides and polysaccharides. More and more evidences show that the interaction between carbohydrates and specific proteins is the core of many important biological processes, including signal transduction, recognition and catalysis, etc. It also plays a major role in development, immunity, infection and cancer. Therefore, these interactions can be used as the target of treatment methods for some diseases, and they also show great potential in the field of food production and processing.

However, protein-carbohydrate interactions are usually transient, and these interactions are quite weak, so in order to compensate for its low affinity, most protein-carbohydrate interactions are multivalent, which makes it difficult and complicated to detect these interactions in vivo and in vitro.

Carbohydrate-protein binding in Concanavalin AFig 1. Carbohydrate-protein binding in Concanavalin A (Fadda, E.; Woods, R.J. 2010)

Services

Creative Proteomics has been researching in the field of protein-carbohydrate interaction for more than ten years and has established a powerful technology platform for analyzing and characterizing the interaction between them. At present, we provide lots of testing and analysis techniques to help scientists more systematically understand and master the various characteristics of the recognition process between carbohydrates and proteins, including X-ray diffraction, nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), surface plasmon resonance (SPR), calorimetric technique, and high-throughput technologies such as glycan microarrays. In addition, our bioinformatics analysts can also provide many quantum chemical calculations and molecular simulations to help customers analyze this interaction process from a variety of different perspectives.

Customers can choose a certain technology according to the needs of the project, or contact us directly for consultation, and our expert team will provide you with a customized experimental program.

Applications

  • Protein-carbohydrate interaction research
  • Drug design and development
  • Biosensor development
  • Tissue engineering material development
  • Research on disease occurrence and progression

Highlights

  • High resolution and high accuracy¬†
  • Combination of experimental methods and calculation methods
  • High sensitivity, able to detect weak interactions
  • Cost-effective

The protein-carbohydrate interaction analysis platform established by Creative Proteomics includes a large number of advanced and mature technologies, which can monitor and analyze relatively weak interactions, and save customers time and money while ensuring high data accuracy. We are honored to be your competent research assistant.

References

  1. Fadda, E.; Woods, R.J. Molecular simulations of carbohydrates and protein-carbohydrate interactions: motivation, issues and prospects. Drug Discovery Today. 2010, 15: 596-609.
  2. Liang, P.H.; et al. Quantitative analysis of carbohydrate-protein interactions using glycan microarrays:  Determination of surface and solution dissociation constants. Journal of the American Chemical Society. 2007, 129(36): 11177-11184.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.