Protein-RNA Interaction Analysis

As a direct product of DNA, RNA is another executor of life activities: involved in protein synthesis, post-transcriptional modification, DNA repair, and gene expression regulation. Non-coding RNA, including long non-coding RNA (lncRNA), small nucleolar RNA (snoRNA) and untranslated mRNA regions, all achieve different functions through direct interaction with RNA binding protein (RBP), such as exons splicing, mRNA export, etc. RNA-protein interaction is the key to cell homeostasis, and interference with this process can lead to many major diseases, including cancer and neurodegenerative diseases. It is speculated that there are about 1,500 proteins in human cells that can bind to RNA to exert its regulatory mechanism. Therefore, systematic research on RNA-protein interactions is essential to elucidate the physiological functions and pathogenic mechanisms of RNA and proteins.

Protein-RNA Interaction Analysis Services We Offer

At Creative Proteomics, we offer lncRNA-protein interaction services to identify proteins that interact with lncRNAs and understand the mechanisms by which these complexes regulate gene expression, protein synthesis, and other biological processes. In addition, a wide range of other RNA-protein interaction services are available, including mRNA-protein interactions, miRNA-protein interactions, tRNA-protein interactions, rRNA-protein interactions, snRNA -protein interactions and snoRNA-protein interactions. We use state-of-the-art technology to provide high-quality data that will help advance your research.

With the development of science and technology, scientists have developed many techniques for studying RNA-protein interactions. At present, these methods can be roughly divided into two categories: methods for detecting proteins bound to the target RNA (RNA-centric) and methods for detecting RNA bound to the target protein (protein-centric). Each method has unique advantages and limitations, so it is very important to choose the best method to solve biological problems.

RNA-RNA binding protein (RBP) interactionFig 1. RNA-RNA binding protein (RBP) interaction (Marx, V. 2018)

Technology Platform for Protein-RNA Interaction Analysis

Creative Proteomics leads you to uncover the intricate interplay between proteins and nucleic acids. Through cutting-edge analysis services, we reveal the mysteries within this delicate realm, offering insights into the pivotal roles of proteins and nucleic acids in biology.

RNA-centric Approach to Discover RNA-Binding Proteins (RBPs)

Discover the Interactome of Your RNA of Interest: Our RNA-centric analysis services focus on unraveling the intricate interactions between your RNA of interest and RNA-binding proteins (RBPs). Through a combination of advanced experimental techniques and computational analysis, we provide comprehensive insights into the regulatory networks driven by these interactions.

Techniques and Services Description
RNA Immunoprecipitation (RIP) Isolate and analyze RBPs bound to your RNA of interest.
ChiRP-MS Service Combine RNA pull-down with mass spectrometry for RBP ID.
Crosslinking and Immunoprecipitation (CLIP) Map RBP binding sites on RNA with high precision.
CLIP-Seq High-throughput sequencing of RNA fragments bound to RBPs.
RNA Pull-Down Service Capture interacting proteins using biotin-labeled RNA probes.
RNA Antisense Purification (RAP) Service Purification and analysis of RNA-RBP complexes using antisense probes.

Protein-centric Approach to Discover RNA Targets

Unveil the RNA Partners of Your Protein: Our protein-centric analysis services focus on identifying the diverse repertoire of RNA molecules that bind to your protein of interest. Through a combination of experimental and computational approaches, we uncover the intricate landscape of protein-RNA interactions.

Techniques and Services Description
Electrophoretic Mobility Shift Assay (EMSA) Assess protein-RNA interactions and binding affinity.
Yeast Three-hybrid (Y3H) Identify RNA molecules that interact with your protein.
Computational RNA-Protein Interaction Prediction Predict potential RNA partners for your protein.
Dual Luciferase Reporter Gene Assay Validate protein-RNA interactions using reporter genes.
Network Analysis Visualize protein-RNA interaction networks within pathways.
Functional Enrichment Analysis Identify biological functions influenced by interactions.

Applications of Protein-RNA Interaction Assay

Deciphering Gene Regulation: Uncover the secrets of post-transcriptional gene regulation, revealing how RNA-binding proteins control mRNA dynamics, translation efficiency, and splicing patterns.

Exploring RNA Localization: Gain a deeper understanding of RNA transport and localization within cells, shedding light on the mechanisms behind subcellular targeting.

Probing RNA Modifications: Investigate the impact of protein-RNA interactions on RNA modifications, influencing RNA structure, stability, and function.

Advancing Viral Studies: Illuminate viral replication strategies and pathogenesis mechanisms by studying interactions between viral RNA and host proteins, paving the way for antiviral strategies.

Unraveling Neurobiology: Gain insights into neuronal development and function, linking protein-RNA interactions to neurodegenerative diseases like Alzheimer's and Parkinson's.

Informing Cancer Research: Explore the role of protein-RNA interactions in cancer biology, offering potential insights into tumor growth, metastasis, and therapeutic targets.

Enhancing Plant Science: Illuminate plant development, stress responses, and defense mechanisms through a deeper understanding of protein-RNA interactions, contributing to crop improvement strategies.

Supporting Structural Studies: Utilize protein-RNA interaction assays to unravel complex structural details, providing crucial information about conformation and binding sites.

Fueling Drug Discovery: Uncover opportunities for drug development by identifying small molecules that modulate specific interactions, potentially leading to novel therapeutic interventions.

At Creative Proteomics, our Protein-RNA Interaction Assay services empower your research with comprehensive insights, driving progress across a spectrum of scientific domains. Join us in unlocking the mysteries of molecular interactions and advancing your quest for knowledge.

Customers can choose a certain technology according to the needs of the project, or contact us directly for consultation, and our expert team will provide you with a customized experimental program.


  • Transcription and post-transcriptional regulation
  • Construction of protein-RNA interaction network
  • Research on disease occurrence and progression
  • Drug discovery and development


  • High resolution and high accuracy
  • Rigorous results, provide complete control
  • Data can be directly used for article publication
  • Multiple technologies to choose from

Creative Proteomics has been focusing on the study of transcription and post-transcriptional regulation for more than ten years and has accumulated a lot of experience in this field. At present, we have built a powerful technology platform with many advanced and mature methods that can help customers comprehensively study protein-RNA interactions. We are honored to be your competent research assistant.


  1. Ramanathan, M.; et al. Methods to study RNA-protein interactions. Nature Methods. 2019, 16(3): 225-234.
  2. Marx, V. Profiling the dress codes of RNA-binding proteins. Nature Methods. 2018, 15(9): 655-658.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.