Protein-RNA Interactions

Protein-RNA Interactions

Background

As a direct product of DNA, RNA is another executor of life activities: involved in protein synthesis, post-transcriptional modification, DNA repair, and gene expression regulation. Non-coding RNA, including long non-coding RNA (lncRNA), small nucleolar RNA (snoRNA) and untranslated mRNA regions, all achieve different functions through direct interaction with RNA binding protein (RBP), such as exons splicing, mRNA export, etc. RNA-protein interaction is the key to cell homeostasis, and interference with this process can lead to many major diseases, including cancer and neurodegenerative diseases. It is speculated that there are about 1,500 proteins in human cells that can bind to RNA to exert its regulatory mechanism. Therefore, systematic research on RNA-protein interactions is essential to elucidate the physiological functions and pathogenic mechanisms of RNA and proteins.

With the development of science and technology, scientists have developed many techniques for studying RNA-protein interactions. At present, these methods can be roughly divided into two categories: methods for detecting proteins bound to the target RNA (RNA-centric) and methods for detecting RNA bound to the target protein (protein-centric). Each method has unique advantages and limitations, so it is very important to choose the best method to solve biological problems.

RNA-RNA binding protein (RBP) interactionFig 1. RNA-RNA binding protein (RBP) interaction (Marx, V. 2018)

Services

Creative Proteomics integrates various excellent techniques for analyzing RNA-protein interactions to form a systematic analysis platform. On this platform, we can help customers complete almost all research on RNA-protein interactions. The technologies we provide include but are not limited to the following content.

  • RNA-centric approach to discover RBP that binds to the RNA of interest

In vitro research methods, these methods can be used to conduct mutation studies to determine the specific RNA nucleotides and protein amino acids involved in a certain RNA-protein pair.

In vivo research methods are helpful for research that depends on the cellular environment. When subcellular localization, RNA/protein modification, or the dynamic range of local protein concentration are a factor, in vivo methods may be best suited to discover and study RNA-protein interactions.

  • Protein-centric approach to discover RNA that binds to the protein of interest

We provide a large number of advanced technologies, including RIP-seq, RNA-seq, and CLIP-seq, which represents an active area of protein-centric method innovation, and its many derivative technologies.

Customers can choose a certain technology according to the needs of the project, or contact us directly for consultation, and our expert team will provide you with a customized experimental program.

Applications

  • Transcription and post-transcriptional regulation
  • Construction of protein-RNA interaction network
  • Research on disease occurrence and progression
  • Drug discovery and development

Highlights

  • High resolution and high accuracy
  • Rigorous results, provide complete control
  • Data can be directly used for article publication
  • Multiple technologies to choose from

Creative Proteomics has been focusing on the study of transcription and post-transcriptional regulation for more than ten years and has accumulated a lot of experience in this field. At present, we have built a powerful technology platform with many advanced and mature methods that can help customers comprehensively study protein-RNA interactions. We are honored to be your competent research assistant.

References

  1. Ramanathan, M.; et al. Methods to study RNA-protein interactions. Nature Methods. 2019, 16(3): 225-234.
  2. Marx, V. Profiling the dress codes of RNA-binding proteins. Nature Methods. 2018, 15(9): 655-658.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.