Microscale Thermophoresis (MST)

Microscale Thermophoresis (MST)

Introduction

Microscale thermophoresis (MST) is a powerful and innovative technique for quantifying molecular interactions. It is highly sensitive to any changes in molecules, so that molecular events can be accurately quantified, regardless of the size or characteristics of the sample being studied. MST can monitor the directional movement of fluorescent molecules through the microscopic temperature gradient in a microliter volume so that it can accurately analyze the movement events in almost any trace solution sample. Since MST accurately measures the interaction by detecting changes in the hydration shell of biomolecules, it avoids the limitations of other fluorescence-based methods. In addition to saving samples, the operation of MST is also very convenient. It can be carried out in almost any buffer, even in plasma and cell lysate. Recently, a method of using the intrinsic fluorescence of tryptophan-containing proteins to follow its thermophoresis has been developed, thereby realizing completely label-free and immobilization-free methods to study molecular interactions. Currently, MST has been widely used to detect the binding of small molecules to proteins, the binding of substrates to enzymes, or the binding of ligands to liposomes.

MST setup and experimentsFigure 1. MST setup and experiments (Jerabek-Willemsena, M.; et al. 2014)

Services

The MST platform established by Creative Proteomics is a mature and excellent technology platform. We introduced Monolith NT.115pico from NanoTemper technologies to provide services to customers, and all operations on this platform are performed by technicians with rich experience and expertise in this technology. The capillary format is used in the whole experiment, and only 4 μl sample is needed to complete the experiment. The experiment must contain a constant concentration of fluorescent probe molecules (such as labeled proteins) and variable concentrations of titrants (such as other proteins, ligands, etc.).

Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Multiple applications of MSFigure 2. Multiple applications of MS (Jerabek-Willemsena, M.; et al. 2014)

Highlights

  • No requirement for immobilization
  • No molecular weight limitation
  • Low sample consumption
  • Short experiment time
  • Wide range of applications, allowing the study of affinity, mechanism of action (MOA), stoichiometry, thermodynamics, stability, aggregation and other fields
  • Excellent selectivity, high accuracy

Creative Proteomics is an international biotechnology company dedicated to research in molecular interactions and other related fields. We have introduced the advanced NanoTemper MST instrument to build a multifunctional and highly sensitive microscale thermophoresis platform that can help customers study various interactions more effectively. Our one-stop service is designed to save customers time and money.

Reference

  1. Jerabek-Willemsena, M.; et al. Microscale thermophoresis: interaction analysis and beyond. Journal of Molecular Structure. 2014, 1077(5): 101-113.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.

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