As drug discovery has become more complex and difficult, it has become increasingly important to use reliable methods for target identification and validation. Target identification and validation, an indispensable procedure, hinges on the identification and validation of drug targets- biological molecules or proteins pivotal in the pathogenesis of diseases. The triumph of drug discovery is inexorably linked to the dependability of target identification and validation- the foundation for the creation of novel therapies.
At Creative Proteomics, we proffer a plethora of avant-garde services tailored to support target identification and validation. Our label-free detection techniques and other innovative technologies bespeak our commitment to providing solutions to an array of therapeutic areas, from cancer to infectious diseases and metabolic disorders. Our skilled team of scientists are here to help you identify and validate targets in a milieu of therapeutic areas.
One of the key techniques we use for target identification is label-free detection. This method allows us to detect biomolecular interactions without the need for labels or tags, providing a more accurate and reliable assessment of protein-protein or protein-ligand interactions. Our label-free detection platform is based on Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI) technologies, which provide high sensitivity, real-time data, and the ability to screen large numbers of potential targets in a short period of time.
Schematic illustration of the basic SPR experiment (1)
Principles of biolayer interferometry (2)
In addition to label-free detection, we also offer a range of other technologies for target identification and validation, including:
Cell-based assays: These assays can be used to identify potential drug targets based on their biological activity in cellular systems. We use a variety of cell lines and primary cells to test the activity of compounds and assess their effects on cell proliferation, differentiation, and apoptosis.
Enzyme assays: We offer a range of enzyme assays to assess the activity of potential drug targets. Our assays can be used to determine enzyme kinetics, substrate specificity, and inhibition by small molecules or biologics.
Biophysical characterization: Our experts can use a range of biophysical techniques, including differential scanning fluorescence (DSF), circular dichroism (CD), and dynamic light scattering (DLS) to characterize the physical properties of proteins and assess their stability, folding, and aggregation.
Our target identification and validation services provide a comprehensive approach to drug discovery, helping our clients identify potential drug targets with high biological relevance and select the most promising candidates for further development.