Protein-protein interaction (PPI) participates in a wide range of biological processes, including metabolism, development, gene regulation, and cell-cell interactions, etc. almost all cellular functions depend on complex PPI. When proteins interact, they usually form stable transient complexes with a series of important functions. Therefore, detecting these PPI is essential to reveal the regulatory mechanisms of individual cells. The importance of PPI has caused them to receive a lot of attention. At present, many new and improved methods have been developed to supplement traditional technologies. Due to research needs, many PPI analyses need to be carried out in living cells, that is, dynamic PPI research, and can be monitored in real-time through easy-to-detect optical signals, which is very valuable for PPI function research.
Fig 1. Schematic diagrams of FRET, BRET, FRET-FLIM (Cui, Y.; et al. 2019)
If researchers need to research the signal transduction from one cell location to another, then traditional biochemical techniques cannot provide enough spatio-temporal information. The given procedures in PPI's in vivo analysis technology are performed on the entire living organism, providing support for dynamic monitoring and analysis of PPI. In the past ten years, Creative Proteomics has vigorously developed in vivo PPI detection technology, fully combining classic methods and emerging techniques to form our technology platform, making the identification and characterization of PPI in vivo easier and more accurate.
Including yeast two-hybrid (Y2H), bacterial two-hybrid (B2H), bioluminescence resonance energy transfer (BRET), fluorescence resonance energy transfer (FRET), bimolecular fluorescence complementation (BiFC), Proximity-dependent Biotin Identification (BioID), split luciferase systems and other technologies.
Including co-localization images such as single-molecule protein proximity index (smPPI), co-tracking of dual particles, and 3D co-localization, etc., which can provide high-level co-localization signals. It also includes single molecule FRET (smFRET), single-molecule protein proximity index (smPPI), FRET-fluorescence lifetime imaging microscopy (FRET-FLIM) etc., all of which can accurately measure PPI in living cells.
Customers can choose a certain technology according to the needs of the project, or contact us directly for consultation, and our expert team will provide you with a customized experimental program.
Protein-protein interactions are extremely important for organisms. Based on this, Creative Proteomics vigorously develops a technology platform for analyzing PPI. While providing many classic methods, we introduce a large number of novel methods to improve the technical system to ensure that customers can conduct experiments systematically, comprehensively and accurately. We are honored to be your competent research assistant.