Biolayer Interferometry (BLI)

Biolayer Interferometry (BLI)

Introduction

Biolayer interferometry (BLI) is a label-free optical technology used to measure intermolecular interactions. It has the advantages of high sensitivity, high throughput, and low vibration/mechanical noise. This technology uses a biosensor with a coated tip (biological layer), immobilizes the bait molecule on the sensor, and then exposes the biosensor tip to buffer conditions and light to monitor the target molecule in real time by recording changes in light interference. The interaction between the analyte and the bait is directly related to the change in the thickness of the biological layer caused by the association of the analyte. The change in the refractive index of the surrounding medium, the change in flow rate, and unbound molecules will not affect the interference pattern. BLI can provide protein binding and quantitative information, as well as kinetic information about the affinity and stability of the interaction.

Biolayer Interferometry (BLI) technology assay principleFigure 1. Biolayer Interferometry (BLI) technology assay principle (Concepcion, J.; et al. 2009)

Services

Biolayer Interferometry (BLI) Platform

Creative Proteomics has been focusing on the research of molecular interactions for nearly two decades. The rich experience and outstanding insights have made us a biotechnology company trusted by researchers all over the world. The BLI technology platform we established is jointly managed by professional BLI technicians and molecular biologists. Our professionalism and dedication have won us a lot of praise.

  • Our platform includes Fort√©BioOctet RED384 and BLItz instruments. Customers can choose specific equipment according to project requirements (sample size, molecular size, speed, etc.). Our professional technicians can guarantee the accuracy of the experiment.
  • This platform can conduct various researches, including protein quantification, kinetic determination, affinity determination, drug/antibody screening, drug development, etc. It can accurately and sensitively provide Ka, Kd ((kinetic binding), KD (equilibrium binding), and other data.

Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Highlights

  • High throughput, high sensitivity
  • Low vibration and mechanical noise
  • Real-time analysis
  • High-throughput analysis of crude samples
  • No microfluidic components: Measure samples directly in the microplate
  • Short experiment period

Creative Proteomics is an international biotechnology company dedicated to the study of intermolecular interactions and other related fields. We have established an advanced biolayer interferometry technology platform that can supplement label-dependent methods to help customers study molecular interactions fast and in real time.

References

  1. Concepcion, J.; et al. Label-free detection of biomolecular interactions using biolayer interferometry for kinetic characterization. Combinatorial Chemistry & High Throughput Screening. 2009, 12: 791-800.
  2. Dzimianski, J.V.; et al. Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry. scientific reports. 2020.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.

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