Biolayer interferometry (BLI) is a label-free optical technology used to measure intermolecular interactions. It has the advantages of high sensitivity, high throughput, and low vibration/mechanical noise. This technology uses a biosensor with a coated tip (biological layer), immobilizes the bait molecule on the sensor, and then exposes the biosensor tip to buffer conditions and light to monitor the target molecule in real time by recording changes in light interference. The interaction between the analyte and the bait is directly related to the change in the thickness of the biological layer caused by the association of the analyte. The change in the refractive index of the surrounding medium, the change in flow rate, and unbound molecules will not affect the interference pattern. BLI can provide protein binding and quantitative information, as well as kinetic information about the affinity and stability of the interaction.
Figure 1. Biolayer Interferometry (BLI) technology assay principle (Concepcion, J.; et al. 2009)
Creative Proteomics has been focusing on the research of molecular interactions for nearly two decades. The rich experience and outstanding insights have made us a biotechnology company trusted by researchers all over the world. The BLI technology platform we established is jointly managed by professional BLI technicians and molecular biologists. Our professionalism and dedication have won us a lot of praise.
Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.
Creative Proteomics is an international biotechnology company dedicated to the study of intermolecular interactions and other related fields. We have established an advanced biolayer interferometry technology platform that can supplement label-dependent methods to help customers study molecular interactions fast and in real time.