Differential Scanning Fluorimetry (DSF)

Differential Scanning Fluorimetry (DSF)

Introduction

Differential Scanning Fluorescence (DSF) is a fast and inexpensive screening method for identifying low molecular weight ligands that bind proteins. It determines the binding events of ligands by measuring the ligand-mediated shifts in the melting temperature of the target protein. When the purified protein is incubated with an environmental fluorescent dye (such as Sypro Orange), its fluorescence is quenched by water. The protein is exposed to a thermal gradient, more and more hydrophobic residues are exposed as the protein unfolds, resulting in increased dye binding and increased signal due to the exclusion of water. The difference in the midpoint temperature in the presence and absence of ligands is closely related to the binding affinity of small molecules, which can be low molecular weight compounds, peptides, or nucleic acids, etc.

Even the binding of low molecular weight ligands can change the stability of the protein, so this method can be used for measurement. At present, differential scanning fluorescence and nanoDSF are widely used biophysical technologies and continue to play a role in various fields (such as drug discovery, fragment screening).

Differential Scanning Fluorimetry (DSF) Platform

Our Services

Creative Proteomics established an improved proprietary system for conventional DSF. Our scientists and technicians are experienced in experiments and data analysis, using our advanced instruments and software to ensure that we provide customers with high-precision experimental results.

In addition, we also provide NanoDSF, which can be used for a wider range of protein samples than conventional DSF, has higher throughput and lower sample consumption than other methods, it is especially suitable for early research and development. It is worth noting that NanoDSF requires that the protein of interest must contain aromatic amino acids (tryptophan or tyrosine).

Differential Scanning Fluorimetry (DSF) Platform

Highlights

  • Screen hundreds of compounds in one hour
  • Environmental dyes allow flexibility in measuring unfolding processes
  • Identify the real binders and exclude pan assay interfering compounds (PAINS)
  • Suitable for almost any type of globular protein, including enzymes, antibodies, and antibody drug conjugates (ADC)

Highlights of NanoDSF

  • Label-free: measure the intrinsic fluorescence of proteins
  • Measure T m, Tonset and Tagg in the same experiment
  • A variety of buffers and viscous solutions can be used

Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Creative Proteomics is an international biotechnology company dedicated to research in molecular interactions and other related fields. The DSF platform we constructed has the characteristics of high sensitivity and efficiency, and the data obtained can be directly used for article publication. Our one-stop service aims to save customers time and money.

Reference

  1. Niesen, F.H.; et al. The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability. Nature Protocols. 2007.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.

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