HDX-MS Epitope Mapping Service

Epitope Mapping is a vital process that helps to identify and locate the specific regions on an antigen that bind to antibodies. This process plays a crucial role in developing targeted therapies and vaccines for various diseases. HDX-MS is a powerful technique that utilizes hydrogen-deuterium exchange to study the protein-protein interaction, protein folding, and protein-ligand binding.

Creative Proteomics offers epitope mapping services using HDX-MS technology, which helps to determine the precise location of antigenic determinants.

Technical advantages of Epitope Mapping by HDX-MS

  • The location of antigenic epitopes can be pinpointed, improving the success rate of drug development.
  • High-resolution structural information can be obtained in a short period of time, accelerating the research process.
  • Low sample consumption and low cost, while being applicable to the analysis of many different types of protein-antibody complexes.

How to use HDX-MS to analyze Epitope Mapping?

HDX-MS epitope mapping is a unique method that allows for the precise identification of antigenic determinants. This process involves the exchange of hydrogen atoms with deuterium atoms in the protein sample. By analyzing the exchange of hydrogen and deuterium, we can determine the rate at which different regions of the protein are exposed to solvent. Regions that are involved in protein-protein interactions are protected from solvent exchange, whereas flexible regions are more exposed to solvent exchange. The data obtained from the HDX-MS experiments can be used to construct a map of the protein that identifies the antigenic determinants.

Why Choose Creative Proteomics's Epitope Mapping Services?

Creative Proteomics is a leading service provider in the field of molecular interactions research, offering a range of high-quality services for drug discovery and development. Our epitope mapping services use state-of-the-art equipment, including high-resolution mass spectrometers and advanced software for data analysis. Our team of experts has extensive experience in HDX-MS epitope mapping and is dedicated to delivering accurate results with fast turnaround times.

Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass SpectrometerQ Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer

Workflow of HDX-MS Epitope Mapping

Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer

Other Services Our HDX-MS Platform provide:

  • Biosimilar characterization
  • Higher-order structure
  • Conformational/Protein dynamics
  • Small molecules interaction (Compound binding analysis)
  • Protein-Protein interactions
  • Protein folding characterization

If you would like to learn more about HDX-MS epitope mapping services or have other needs, please contact us. We look forward to cooperating with you.

* This service is for RESEARCH USE ONLY, not intended for any clinical use.