Protein-Fragment Complementation Assay (PCA)

Protein-Fragment Complementation Assay (PCA)

Introduction

Protein-Fragment Complementation Assay (PCA) is a well-suited method for detecting protein interactions in the cells. This method is based on the fusion of the hypothetical binding partners to two rationally designed fragments of a reporter protein. The interaction between the bait and the prey protein brings the split reporter fragments together, enabling them non-covalent and specific reassembly, thereby rebuilding their native structure and activity. Reporter proteins are usually those that can generate detectable signals, such as fluorescence, luminescence, colorimetric signals, and survival selection analysis.  

Because of its high sensitivity, high signal-to-background ratio, and complete reversibility, PCA has been widely used. In addition to being used for different levels of protein-protein interaction research (ranging from bacteria to animals or plants), the PCA provided by Creative Proteomics can also be used for biochemical network research, drug effects identification, PPIs inhibitors screening, etc.

High-throughput application of protein-fragment complementation assays (PCAs) to study protein interactionsFigure 1. High-throughput application of protein-fragment complementation assays (PCAs) to study protein interactions (Morell, M.; et al. 2009)

Services

Protein-Fragment-Complementation-Assay-(PCA)-2

Creative Proteomics has recruited many professional technicians and experts to jointly build an advanced PCA technology platform. At present, we have accumulated a lot of PCA-related experience, and have provided high-quality, cost-effective PCA research to customers from all over the world. In order to study the interactions between different specific proteins, fluorescent or luminescent proteins are chosen as reporters, which can be more easily imaged in vivo. In addition, we also provide bimolecular fluorescence complementation-based resonance energy transfer (BIFC-RET) and other technologies to help customers more systematically. In our experiments, specific strategies will be made according to different situations, different aspects must be considered:

  • Protein reporter
  • Protein reporter fragments
  • Linker
  • Topology of the protein fusion
  • Expression system
  • Controls

Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Highlights

  • Direct detection of molecular interactions in vivo and in vitro
  • Reversible, can analyze complex dynamics
  • Wide range of research, including PPI in any cell line and multicellular organisms
  • High-throughput screening
  • Capture perturbation of interactions, such as drugs, hormones, gene knockouts, or environmental induction
  • High flexibility and sensitivity

Creative Proteomics is an international biotechnology company dedicated to research in molecular interactions and other related fields. The protein-fragment complementation assay platform we constructed has the characteristics of high quality and efficiency, and the data obtained can be directly used for paper publication. Our one-stop service aims to save customers time and money.

References

  1. Morell, M.; et al. Protein complementation assays: Approaches for the in vivo analysis of protein interactions. FEBS Letters. 2009.
  2. Michnick, S.W.; et al. Detection of protein-protein interactions by protein fragment complementation strategies. Method. Enzymol. 2000.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.

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