Protein Microarray

Protein Microarray

Introduction

Microarray technology is to perform thousands of detections on a miniaturized device and was first proposed by Roger Ekins in 1989. This concept was first successfully transformed into DNA microarray technology, which can measure the mRNA expression levels of thousands of genes in parallel. However, DNA microarray technology has unavoidable limitations because the mRNA profile is not always correlated with protein expression. Since protein is the major driving force of almost all cellular processes, protein microarrays have been developed as a high-throughput tool for research in various fields.

Protein microarrays can be divided into two classes, analytical and functional, which have the characteristics of high sensitivity and good reproducibility. Unlike antibody arrays (analytical microarrays), functional protein microarrays are made by spotting all proteins encoded by an organism, so they can be used to study protein functions, including protein-other molecule interactions, enzyme-substrate relationship, biochemical activities, and immune responses, etc.

Schematic representation of protein microarrayFigure 1. Schematic representation of protein microarray (Baharvand, H.; et al. 2007)

Services

Protein-Microarray-Platform-2

The protein microarray platform Creative Proteomics sets up is jointly responsible by experienced protein analysts and experts. The platform is updated on time and upgraded regularly to provide customers with first-class, high-quality protein-related analysis services. There are still many challenges in the process of protein microarray services, especially in the production of proteins in a high-throughput fashion. The key points of our services include but are not limited to the following aspects:

  • Protein production: a large amount of highly purified proteins
  • Surface chemistry: immobilize proteins and preserve their three-dimensional conformation, providing many different characteristics of surfaces and immobilization methods
  • Detection: label dependent and label free, and we also provide a variety of other technologies such as atomic force microscopy (AFM) and MALDI-TOF MS for technological expansion.

Customers can choose different technology platforms according to project requirements, or contact us directly for consultation, and our expert team will provide you with customized experimental procedures.

Application

  • Analysis of protein binding properties, including the interaction of proteins with proteins, peptides, nucleic acids, small molecules, glycans, etc.
  • Monoclonal antibody specificity profiling
  • Analysis of protein post-translational modifications, such as protein phosphorylation, ubiquitylation, acetylation, etc.
  • Research on host-microbe interactions
  • Biomarker identification

Creative Proteomics is an international biotechnology company dedicated to research in molecular interactions and other related fields. The protein microarray platform we constructed has the characteristics of high quality and efficiency, and the data obtained can be directly used for paper publication. Our one-stop service aims to save customers time and money.

References

  1. Sutandy, F.X.R.; et al. Overview of Protein Microarrays. Curr Protoc Protein Sci. 2013.
  2. Baharvand, H.; et al. Concise Review: Trends in Stem Cell Proteomics. Stem Cells. 2007.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.

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