Protein-Protein Interaction in Vitro Analysis

Protein-Protein Interaction in Vitro Analysis

Background

Protein interaction refers to a process in which two or more protein molecules combine to form a protein complex. Under normal circumstances, it is difficult for a protein to function alone, it is the coordination of multiple protein molecules to realize complex cell functions. Therefore, research focusing on protein interaction is more conducive to exploring the occurrence and development of diseases, finding specific disease treatment methods, and providing new ideas in the research of new drug development.

Due to its significance, a large number of emerging technologies have been developed in recent years to supplement and perfect the classical methods. Each technology has its advantages and disadvantages in a specific environment. Creative Proteomics integrates lots of advanced technologies and combines them to provide customers with the best quality service.

Different levels of characterization of protein interactions in vivo and in vitroFig 1. Different levels of characterization of protein interactions in vivo and in vitro (Piehler, J. 2005)

Services

There are many methods for in vitro analysis of protein-protein interactions. Creative Proteomics provides comprehensive in vitro analysis services for PPI, such as tandem affinity purification (TAP), native PAGE, surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), biolayer interferometry (BLI), microscale thermophoresis (MST) and ELISA-based assays.

Our technology platform also includes protein fragment complementation assay (PCA), which is one of the most extensive methods for characterizing PPI or identifying new chaperones in vivo and in vitro, as well as phage display, X-ray crystallography, NNR and protein microarrays, etc.

In subsequent experiments, Creative Proteomics also provides liquid chromatography-mass spectrometry (LC-MS) for identification and analysis of protein or protein mixtures in purified samples purified from IP, Co-IP, and pull-down experiments.

Customers can choose a certain technology according to the needs of the project, or contact us directly for consultation, and our expert team will provide you with a customized experimental program.

Applications

  • Protein-protein interaction research
  • Protein function research
  • Proteomics research
  • Disease mechanism research
  • Discover drug targets
  • Inhibitor screening

Highlights

  • High throughput and high resolution
  • Highly responsive, even the weakest interaction in proteins can be detected
  • Multiple methods of cross-checking to ensure accurate results
  • Unparalleled technology platform
  • Experienced researchers

The importance of protein-protein interaction has prompted scientists to develop lots of techniques for studying it in vitro. Creative Proteomics has established a huge technology platform, which includes a large number of advanced and continuously upgraded methods to ensure to provide customers with high-quality, high-accuracy analysis services. We are honored to be your competent research assistant.

References

  1. Rao, V.S.; et al. Protein-Protein Interaction Detection: Methods and Analysis. International Journal of Proteomics. 2014: 1-12.
  2. Piehler, J. New methodologies for measuring protein interactions in vivo and in vitro. Current Opinion in Structural Biology. 2005, 15(1): 4-14.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.