Protein-Natural Product Interactions

Protein-Natural Product Interactions

Background

At present, natural products have become an important source of new drugs for various diseases. The interaction between natural products and intracellular protein targets is the basis for their pharmacological activities. According to statistics, in the past few decades, about 50% of the drugs approved by the FDA are derived from natural products, collectively referred to as natural drugs. They have been widely used in the treatment of many diseases such as cancer, for example, the natural plant alkaloid elliptinium was developed as an anti-cancer natural medicine Celiptium. Compared with chemically synthesized drugs, natural drugs derived from natural products have significantly reduced side effects, novel structures, enhanced biocompatibility and functional diversity.

However, the development of new drugs based on natural products is a long and expensive process, and most drugs interact with multiple protein targets instead of a single target. Therefore, this multi-target recognition mode makes it challenging to study protein-natural product interactions. Creative Proteomics integrates bioinformatics, mass spectroscopy, proteomics and metabolomics and other disciplines to form an unparalleled technology platform to provide support for the vast number of researchers in the study of protein-natural product interactions.

Rosuvastatin is a natural-product-inspired drugFig 1. Rosuvastatin is a natural-product-inspired drug (Rodrigues, T.; et al. 2016)

Services

Creative Proteomics integrates a variety of technologies to form a systematic protein-natural product interaction analysis platform. The services we provide include but are not limited to the following technologies.

  • Activity-based protein profiling (ABPP)

Probe design and synthesis – Target protein binding – Target protein analysis – Biological function determination by SPR, MST, ITC, etc.

  • Compound-centric chemical proteomics (CCCP)

Immobilize the drug molecule on the matrix (agarose beads, magnetic beads, etc.) – Incubate – Elute – Identify

Immobilize a variety of proteins on a high-density microarray, label the molecules to be tested with affinity tags (fluorophores, biotin, photoaffinity groups, radioisotopes, etc.). This method has the advantage of high throughput, but the label tags may affect activity.

Customers can choose a certain technology according to the needs of the project, or contact us directly for consultation, and our expert team will provide you with a customized experimental program.

Applications

  • Protein-natural product interaction research
  • Discovery and development of new natural medicines
  • Research on the mechanism of action (MOA) of natural products

Highlights

  • Simple and efficient
  • Reliable results with minimal false positives
  • High throughput
  • Multiple technologies to choose from

The basis for natural products to function in cells is their interaction with target proteins. Based on this, Creative Proteomics’ technicians with rich experience in this field can provide customers with a variety of efficient and high-throughput technologies to help scientists explore the interaction between natural products and proteins. We are honored to be your competent research assistant.

References

  1. Chen, X.; et al. Target identification of natural medicine with chemical proteomics approach: probe synthesis, target fishing and protein identification. Signal Transduction and Targeted Therapy. 2020.
  2. Rodrigues, T.; et al. Counting on natural products for drug design. Nature Chemistry. 2016, 8(6): 531-541.
* This service is for RESEARCH USE ONLY, not intended for any clinical use.